Artemisia annua is an annual herb belonging to the genus Artemisia in the Asteraceae family. The whole plant possesses traditional medicinal properties, including clearing heat, relieving summer heat, cooling the blood, and promoting diuresis. It is also the natural source of artemisinin, a highly effective antimalarial drug. In recent years, with the successful mapping of the Artemisia annua genome at the chromosomal level and the establishment of an efficient genetic transformation system, key breakthroughs have been achieved in the functional genomics research of this species.

Lifeasible specializes in genetic transformation services for Artemisia annua. Leveraging a mature Agrobacterium-mediated transformation method combined with gene editing technologies, we have established a highly efficient and reliable technical platform for clients to elucidate the artemisinin biosynthetic pathway and regulatory networks. This service accelerates the directed molecular breeding of new varieties with high artemisinin content and lays a systematic foundation for using Artemisia annua as a "bioreactor" to produce high-value-added products.

Our Service for Artemisia annua Genetic Transformation

Experimental principle

We use Artemisia annua leaves as the recipient material for genetic transformation. We infect the recipient material with Agrobacterium carrying the target gene vector to insert the T-DNA into the genome. Following antibiotic selection for the corresponding resistance, we obtain independent resistant callus tissue, which is further differentiated and regenerated into transgenic lines.

Agrobacterium-mediated stable transformation

The Agrobacterium-mediated genetic transformation method enables the stable integration and inheritance of exogenous genes. The basic procedure involves selecting explants such as petioles or leaves, infecting them with Agrobacterium strains containing the target gene, and, following co-culture, inducing callus formation and plant regeneration on a medium containing screening antibiotics and bactericides.

Agrobacterium-mediated transient transformation

To rapidly validate gene function, we have established a highly efficient transient transformation system using leaves from Nicotiana benthamiana or Artemisia annua. For example, constructed vectors are introduced into leaves via Agrobacterium-mediated infiltration to detect reporter gene expression, thereby assessing promoter activity or the gene's transient effects.

Our Service Workflow

Fig.2 Our service process. (Lifeasible)

Highlights of Our Services

• Guaranteed project success rates. With years of technical expertise in plant genetic transformation and a highly experienced professional team, we ensure efficient experiments and high success rates.
• Efficient experimental workflow. Clients need only provide Artemisia annua seeds or plants to initiate the project quickly. We employ an optimized Agrobacterium-mediated transformation method that is fast and efficient, primarily resulting in single-copy insertions and supporting various eukaryotic resistance screens.
• Comprehensive service support. We provide full-service support, including preliminary protocol guidance and assistance in optimizing experimental design. Throughout the process, clients are kept informed of project progress. Upon completion, we deliver reliable, high-quality experimental results as stipulated in the contract, including positive seedlings and relevant testing reports.

Lifeasible specializes in providing plant genetic transformation services. We stay at the forefront of advancements in plant genetic transformation, offering advanced technical support and reliable solutions for the genetic transformation of Artemisia annua. We empower researchers to increase artemisinin content and achieve stable, high-efficiency production specifically. If you are interested, please feel free to contact us.