Product Name
GFP | Green Fluorescence Protein (affinity purified)
Immunogen
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Quantity
100 µg at 1 mg/ml
Reactivity
Native and Recombinant GFP
Confirmed reactivity
Native GFP, Recombinant GFP (E.coli), all variants of GFP, including EGFP
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 