Product Name
FITC | fluorescein isothiocyanate, Biotin-conjugated (monoclonal, Clone NI 239)
Immunogen
Highly purified fluorescein isothiocyanate isomer 1.
Background
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Purity
Purified IgG.1 in PBS pH 7.2, biotin conjugated.
Confirmed reactivity
FITC molecule
Reconstitution
For reconstitution add 0.5 ml of sterile water
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
Recommended dilution
The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
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Datasheet 