Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
Product Name
Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
Immunogen
Purified Rat IgG, whole molecule
Background
Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Purity
Immunogen affinity purified goat IgG.
Confirmed reactivity
Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins
Predicted reactivity
Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Storage
Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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Datasheet 