Product Name
ARF1 | ADP-ribosylation factor 1
Immunogen
Recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, AT2G47170)
Background
The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.
Reactivity
A. thaliana, C.reinhardtii, Elaeis sp. , L. longiflorum, M. truncatula, N. tabacum, O. sativa, P. hybrida cv Mitchell, P. patens, S. tuberosum
Confirmed reactivity
Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp. , Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum
Predicted reactivity
Brassica juncea, Brassica napus, Capsella rubella, Capsicum annum, Cucumis sp., Daucus carota, Elaeis guineensis, Glycine max, Helleborus orientalis, Hordeum vulgare, Medicago truncatula, Nannochloropsis gaditana, Nicotiana benthamina, Ostreococcus tauri, Populus trichocarpa, Triticum aestivum, Zea mays
Not reactive in
Microsporidia sp.
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
21 kDa (Arabidopsis thaliana)
Tested applications
Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Recommended dilution
1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 