Product Name
BiP | Lumenal-binding protein (chicken antibody)
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 UniProt:Q9LKR3, TAIR: At5g28540, BiP2 UniProt: F4K007, TAIR: At5g42020, BiP3 UniProt:Q8H1B3 ,TAIR:At1g09080
Background
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Purity
Immunogen affinity purified total IgY. in PBS pH 7.4.
Reactivity
Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea maysLimited stock
Confirmed reactivity
Arabidopsis thaliana, Hordeum vulgare, Physcomitrium patens, Spinacia oleracea, Zea mays
Predicted reactivity
Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
73.5 | 80 kDa
Tested applications
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Storage
Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 