Product Name
Goat anti-Rabbit IgG (H&L), ALP conjugated
Immunogen
Purified Rabbit IgG, whole molecule
Background
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Format
Liquid, clear, colorless.
Purity
Immunogen affinity purified goat IgG.
Confirmed reactivity
Rabbit IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 500-1: 8 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 500-1: 8 000 (WB)
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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Datasheet 