Product Name
H3 | Histone H3 (rabbit antibody) (nuclear marker)
Immunogen
KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)
Background
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Reactivity
A. thaliana, C. reinhardtii, H. vulgare, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays
Confirmed reactivity
Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Phalaenopsis equestris, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
Predicted reactivity
Brachypodium distachyon, Brassica napus, Emiliania huxleyi, Glycine max, Nannochloropsis gaditana, Nicotiana tabacum, Malus domestica, Medicago sativa, Oryza sativa, Ostreococcus sp. , Pinus pinaster, Pisum sativum, Porphyridium purpureum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
15 | 17 kDa
Tested applications
ChIp-qPCR (ChIp-qPCR), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 100-1 : 500 (ICC), 2 µl of antibody/500 µl solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 