Product Name
PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)
Immunogen
Native purified 33 kDa protein from Spinacia oleracea UniProt: P12359
Background
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Reactivity
A.thaliana, C. reinhardtii, C. vulgaris, C. sp. DT, E. crus-galli, F. arundinacea cv. Kord, H. pluvialis, H. spontaneum, H. vulgare, N. benthamiana, P. abies, P. banksiana, P. sativum, P. miliaceum, S. oleracea, S. tuberosum cultivar Taedong Valley, Synechococcus sp. PCC 7942 and PCC6803, T. salsuginea,T.aestivum compartment marker of thylakoid lumen
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Chlorella vulgaris, Echinochloa crus-galli, Eucalyptus grandis x Eucalyptus camaldulensis, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC7002, sp. PCC 7942 and sp. PCC6803, Thellungiella salsuginea, Triticum aestivum, Zea mays
Predicted reactivity
Glycine max, Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Zosteria marina
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
35 | 33 kDa
Tested applications
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 