Product Name
Tubulin alpha chain, Biotin conjugated (clone TU-01)
Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36
Background
Tubulin alpha (TUA) together with beta tubulin is making up microtubules. The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.
Format
Liquid at 1 mg/ml.
Purity
Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin
Reactivity
Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
Confirmed reactivity
Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
Expected/apparent MW
50 | 54 kDa
Tested applications
Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)
Recommended dilution
1-2 μg/ml (WB)
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 