Product Name
Tubulin alpha chain (polyclonal antibodies)
Immunogen
KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139(At1g64740), alpha-2/alpha-4 chain B9DGT7(At1g50010), alpha-5 chain B9DHQ0(At5g19780), alpha-6-chain P29511(At4g14960)Peptide used to elict this antibody is not present in tubulin beta.
Background
Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
Reactivity
A. thaliana, C. reinhardii, E. gracilis, H. vulgare, O. sativa, Setaria italica, Zea mays
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardii, Euglena gracilis, Hordeum vulgare, Oryza sativa, Setaria italica, Zea mays
Predicted reactivity
Brassica napus, Chlorella vulgaris, Chlorella variabilis, Cucumis sativus, Euglena gracilis, Glycine max, Micromonoas pusilla, Nannochloropsis gaditana, Ostreococcus lucimarinus, Pisum sativum, Physcomitrium patens, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Triticum aestivum, Vigna radiata, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
49 | 52 kDa (Arabidopsis thaliana)
Tested applications
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 500 (IF), 1 : 1000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 