Product Name
RA | Rubisco activase
Immunogen
Purified, recombinant Rubisco activase from Gossypium hirsutum Q9AXG1
Background
RA - Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA.
Reactivity
A. thaliana, Caesalpinia pulcherrima, C. sativa, C. reinhardtii, H. spontaneum, F. pRatensis, G. max, G. hirsutum, G. barbadense, L. perenne, N. oceanica, N. tabacum, O. sativa, P. balsamifera, R. discolor, S. lycopersicum, Z. mays, T. salsuginea, red sulfur bacterium T. sp. Cad16 (isolated from Lake Cadagno)
Confirmed reactivity
Arabidopsis thaliana, Camelina sativa, Chlamydomonas reinhardtii, Caesalpinia pulcherrima, Hordeum spontaneum, Festuca pratensis, Glycine max, Gossypium hirsutum, Gossypium barbadense, Kalanchoë fedtschenkoi, Lolium perenne, Nannochloropsis oceanica, Nicotiana tabacum, Oryza sativa, Populus balsamifera, Rhoeo discolor, Solanum lycopersicum, Zea mays, Thellungiella salsuginea, red sulfur bacterium Thiodictyon sp. Cad16 (isolated from Lake Cadagno)
Predicted reactivity
Glycine max, Gossypium mexicanum, Hordeum vulgare, Medicago sativa, Olea europea, Picea sitcHensis, Physcomitrium patens, Ricinus communis, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in
marine picocyanobacteria
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
47 and 42 kDa (maize, tobacco, Chlamydomonas)
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000-1 : 10 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 