Product Name
PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)
Immunogen
native, purified 23 kDa protein from Spinacia oleracea,UniProt: P12302
Background
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Reactivity
A. thaliana, Ch. reinhardtii, P. banksiana. , S.oleracea, T. aestivum
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Predicted reactivity
Musa acuminata, Oryza sativa, Pinus monticola, Pisum sativum, Populus balsamifera, Solanum lycopersicum
Not reactive in
Synechococcus sp. PCC 7942
Reconstitution
For reconstitution add 200 µl of sterile water
Expected/apparent MW
28 | 23 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 