Product Name
COXII | Plant Cytochrome oxidase subunit II (affinity purified)
Immunogen
KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
Background
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
Eudicots, monocots, Physcomitrella patens
Confirmed reactivity
Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra
Predicted reactivity
Cucumis melo, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Triticum aestivum, Vigna radiata
Not reactive in
Saccharina japonica
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
29.4 | 30 kDa (for Arabidopsis thaliana)
Tested applications
Blue Native-PAGE (BN-PAGE), Western blot (WB)
Recommended dilution
1 : 1000 (BN-PAGE), 1 : 1000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 