Product Name
Cyt c | Cytochrome c
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
Background
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
A. thaliana, B. oleracea, G. max, L. luteus, P. satiovum, Z. mays
Confirmed reactivity
Arabidopsis thaliana, Brassica oleracea, Glycine max, Pisum sativum, Zea mays
Predicted reactivity
cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrium patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera.
Not reactive in
Arabidopsis thaliana CytC6, Chinesecuscuta sp.
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
12.5 | 14 kDa (for Arabidopsis thaliana)
Tested applications
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1: 100 (IL), 1 : 5000 (WB)
Storage
Store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 