Product Name
DISCONTINUED COXII | Cytochrome oxidase subunit II (200 µg)
Immunogen
KLH-conjugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativaP04373 and Physcomitrella patens Q1XGA9
Background
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
Eudicots, monocots, Physcomitrella patens
Confirmed reactivity
Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica
Predicted reactivity
Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 130 µl of sterile water
Expected/apparent MW
29.4 | 30 kDa (for Arabidopsis thaliana)
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 