Product Name
COXII | Cytochrome oxidase subunit II (plant)
Immunogen
KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
Background
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Purity
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02% sodium azide.
Reactivity
A. thaliana (leaf extract and isolated mitochondria), B. oleracea, P. major, P. euryphylla, S. uniflora, S. dioica
Confirmed reactivity
Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Physcomitrium patens, Silene uniflora, Silene dioica
Predicted reactivity
Dicots including Cannabis sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
29.4 | 30 kDa (for Arabidopsis thaliana)
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Storage
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 