Product Name
HSP70 | Heat shock protein 70 (chloroplastic)
Immunogen
KLH-conjugated synthetic peptide derived from higher plant chloroplastic HSP70, inluding Arabidopsis thaliana cpHSC70-1, At4g24280 and cpHSC70-2, At5g49910
Background
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Reactivity
A. thaliana, H. vulgare, O. sativa, P. sativm, P. strobus
Confirmed reactivity
Arabidopsis thaliana, Brassica napus, Hordeum spontaneum, Hordeum vulgare, Oryza sativa, Pinus strobus, Pisum sativum
Predicted reactivity
Arundo donax, Brachypodium distachyon, Brassica rapa subsp. pekinensis, Brassica napus, Capsella rubella, Citrus clementina, Citrus sinensis, Coffea canephora, Glycine max, Glycine soja, Hordeum vulgare, Medicago trancatula, Oryza sativa, Phaseolus vulgaris, Physomitrella patensm, Picea sitchemsis, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum tuberosum, Solanum lycopersicum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 200 µl of sterile water
Expected/apparent MW
76 | 70 kDa
Tested applications
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 100 (IP), 1 : 2000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 