Immunogen
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Background
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Purity
Purified IgG in PBS.
Reactivity
Canavalia ensiformis (common jack-bean)
Confirmed reactivity
Canavalia ensiformis
Predicted reactivity
Arabidopsis thaliana, Glycine max, Ricinus communis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Expected/apparent MW
91 kDa
Tested applications
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Recommended dilution
1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 