Product Name
Fd-GOGAT | Ferredoxin-dependent Glutamate synthase
Immunogen
Purified full length, tag cleaved, recombinant Zea mays GOGAT, UniProt: P23225
Background
Glutamine oxoglutarate aminotransferase (GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 in Arabidopsis. Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria. Ferredoxin-dependent glutamate synthase, chloroplastic (Fd-GOGAT) is involved in glutamate biosynthesis in leaf. This protein required for the reassimilation of ammonium ions generated during photorespiration. Gene name is GlsF.
Format
Liquid at 2 mg/ml.
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Reactivity
Arabidopsis thaliana, Spinacia oleracea, Synechocystis sp. PCC6803, Zea mays
Confirmed reactivity
Arabidopsis thaliana, Spinacia oleracea, Synechocystis sp. PCC6803, Zea mays
Predicted reactivity
Arthrospira platensis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
175 kDa (Zea mays), 168 kDa (Arabidopsis thaliana)
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 2000 - 1: 5000 (WB)
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 