Product Name
NodGS | Nodulin / glutamate-ammonia ligase-like protein (affinity purified)
Immunogen
KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180
Background
NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment. It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.
Purity
Immunogen affinity purified serum in PBS pH 7.4. with glycerol at 50%.
Reactivity
Arabidopsis thaliana
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Medicago truncatula, Theobroma cacao
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
94 | 90 kDa
Tested applications
Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 