Product Name
GLN2 | GS2, chloroplastic form of glutamine synthetase
Immunogen
KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630
Background
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
Reactivity
A. thaliana, N. tabacum, O. sativa, P. sativum, S. oleracea
Confirmed reactivity
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea
Predicted reactivity
Brassica napus, Diplotaxis tenuifolia,Eruca versicaria, Glycine max, Hordeum vulgare, Medicago truncatula, Pinus sylvestris, Phaseolus vulgaris, Physcomitrium patens, Populus sp., Triticum aestivum, Zea mays
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
47 | 44-45 kDa
Tested applications
ImmunoGold (IG), (Western blot (WB)
Recommended dilution
1 : 5000 on 0.5-5 ug protein/lane detection (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 