Product Name
PM Peptone Bromide Agar Base
Product Overview
Phytopathogenic Microbe peptone bromide agar base is semi selective medium for the detection of Xanthomonas hortorum pv. carotae in carrot.
Form
Cream to yellow coloured, homogeneous, free flowing powder
Characteristic
Cultural characteristics observed with added CNF Supplement (PHS006), after an incubation at 25- 30 centigrade for 5-7 days.
Reaction of 3.53 % w/v aqueous solution is pH 7.4 ± 0.2 at 25 centigrade.
Storage
Store below 30 centigrade and the prepared medium at 2 - 8 centigrade.
Instructions
Suspend 35.3 grams in 900 ml distilled water containing 10 ml of Tween 80. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Dissolve 10.0 grams of skim milk powder in 100 ml of distilled water. Sterilize by autoclaving at 15 lbs pressure (121 centigrade) for 5 minutes. Mix Part A and Part B, cool to 45-50 centigrade.and aseptically add the rehydrated content of one vial of CNF supplement. Mix well and pour into Petri plates.
Datasheet 