3' Primer
T7 Reverse GCTAGTTATTGCTCAGCGG
5' Primer
SP6 Forward ATTTAGGTGACACTATAG
Cloned Strain(s)
ccdB positive screening DB3.1 competent cells, negative screening with OmniMAX2-T1 competent cells
Host cells (lines)
Conventional cell lines, 293, CV-1, CHO, etc.
Stable
Transient or stable expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian cell expression vectors