Product Name
PM Tyrosine Peptone Agar Base (MT Medium)
Product Overview
Phytopathogenic Microbe tyrosine peptone agar base (MT Medium) is semi selective medium for the detection of Pseudomonas savastanoi pv. phaseolicola, Pseudomonas syringae and Xanthomonas axonopodis in seeds of beans.
Form
Cream to yellow coloured, homogeneous, free flowing powder
Characteristic
Cultural characteristics observed with added CNVV Supplement, after an incubation at 25-30 centigrade for 5- 6 days.
Storage
Store below 30 centigrade and the prepared medium at 2 - 8 centigrade.
Instructions
Suspend 25.75 grams in 890 ml distilled water containing 10 ml of Tween 80. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 centigrae) for 15 minutes. Dissolve 10 grams of skim milk powder in 100 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121 centigrade) for 5 minutes. Cool to 45 - 50 centigrade. Aseptically mix both the solution and add the rehydrated contents of one vial of CNV supplement. Mix well and pour into sterile Petri plates.
Datasheet 