Product Name
Potato Dextrose Agar
Product Overview
Potato Dextrose agar is recommended for the isolation and enumeration of yeasts and moulds from dairy and other food products.
Form
Cream to yellow coloured granular media
Characteristic
Cultural characteristics observed after incubation at 20-25 centigrade for 2-5 days. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose Agar.
Reactionof 3.9% w/v aqueous solution at 25 centigrade (after sterilization).pH:-5.6±0.2.
Storage
Store below 30 centigrade in tightly closed container and the prepared medium at 2 - 8 centigrade.
Instructions
Suspend 39 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Cool to 45-50 centigrade. Mix well and pour into sterile Petri plates or tubes as desired. before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.
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