35S-pat GMO Screen Kit (24)

GMO-100

35S-pat GMO Screen Kit (24)

This is a real-time PCR-based detection kit of 35S-pat construct in food, animal feed, and pharmaceutical products.

The 35S-pat GMO Screen Kit (24) is designed for the target-specific detection of the 35S-pat construct DNA in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol.

GMO Detection Kit are shipped on dry ice. Multiplex PCR Master Mix and ROX dye should be stored immediately at –20°C upon receipt, in a constant-temperature freezer. All remaining not reconstituted kit components should be stored at 2–8°C and protected from light. Stored under these conditions and handled correctly, assay performance remains unaffected until the date of expiration printed on the quality control label inside the kit box or envelope. Reconstituted reagents of GMO Detection Kit should be dispensed into aliquots to avoid more than 5 freeze–thaw cycles, and stored at 2–8°C for short-term storage (1 month) or –20°C for long-term storage.

PCR Assay Screen 35S-pat;
Internal Control;
Positive Control;
Multiplex PCR Master Mix;
Nucleic Acid Dilution Buffer;
RNase-free water;
50x ROX Dye Solution.

The 35S-pat GMO Screen Kit (24) is designed for the qualitative detection of genetically modified organisms containing the pat gene, driven by the 35S promoter. The expression of the pat gene mediates tolerance to the herbicide phosphinotricin in crops.
Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

Real-time PCR

Highly sensitive and specific detection of GMOs with the 35S-pat construct;
Streamlined detection protocol;

Food