Product Name
Ammonia Colorimetric Assay Kit
Product Overview
This kit provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In the assay, Ammonia is converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of ammonia, much more sensitive than measuring NADPH based ammonia assay.
Description
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.
Applications
The kit can detect 1 nmol (~20 μM) of ammonia, much more sensitive than measuring NADPH based ammonia assay.
Kit Components
• Ammonia Assay Buffer
• OxiRed Probe in DMSO
• Enzyme Mix (lyophilized)
• Developer
• Converting Enzyme (Lyophilized)
• NH₄Cl Standard (100 mM)
Detection method
Absorbance (570 nm)
Datasheet 