Creatine Colorimetric/Fluorometric Assay Kit

AgK-322

Creatine Colorimetric/Fluorometric Assay Kit

Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001 - 10 mM Creatine.

Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.

The kit can detect 0.001 – 10 mM Creatine.

Store at -20°C.

100 assays

• Creatine Assay Buffer
• Creatine Probe
• DMSO (Anhydrous)
• Creatine Enzyme mix
• Creatine Standard (10 µmol)

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)