Product Name
Formate Colorimetric Assay Kit
Product Overview
In the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.
Description
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
Applications
The assay can detect formate in the range from 0.5 to 10 nmols/well.
Kit Components
• Formate Assay Buffer
• Formate Enzyme Mix
• Formate Substrate Mix
• Formate Standard (100 mM)
Detection method
Absorbance (450 nm)
Datasheet 