Product Name
Fructose Assay Kit (Colorimetric)
Product Overview
Fructose Assay Kit utilizes an enzymatic mechanism by which a highly specific enzyme metabolizes fructose, and then a developer is used to generate the proportional colorimetric signal that can be quantified at 570 nm. The assay shows greater than 100-fold specificity for Fructose over glucose and other sugars, both mono- and polysaccharides. The method is suitable for use in a range of biological and culinary samples, and can detect as lower than 0.1 nmole (~15 ng) Fructose.
Description
Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose, although it has a much lower glycemic index than both sucrose and glucose. For these reasons, fructose has been investigated as a food sweetener, and its downstream metabolic effects are of considerable interest. Studies have shown that fructose levels may correlate with cardiometabolic disease, gout, and liver function.
Applications
Rapid, specific assessment of fructose levels in biological samples. Glucose does not interfere in the detection of fructose
Kit Components
• Fructose Assay Buffer
• Fructose Development Buffer
• Fructose Enzyme
• Fructose Substrate
• Fructose Developer
• Fructose Assay Buffer
• OxiRedTM Probe (in DMSO)
• Fructose Standard (100mM)
Materials Required but Not Supplied
Plate Reader capable of 37°C setting and absorbance readings
96-well clear plate with flat bottom
Detection method
Absorbance (OD: 570 nm)
Compatible Sample Types
Cell and Tissue Lysates
Food and Beverage Samples
Datasheet 