Lactose Colorimetric/Fluorometric Assay Kit

AgK-361

Lactose Colorimetric/Fluorometric Assay Kit

In the Lactose Assay Kit, Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (O.D 570 nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.

Lactose (C₁₂H₂₂O₁₁ FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

N/A

Store at -20°C.

100 assays

• Lactose Assay Buffer
• Probe (DMSO solution)
• Lactase (Lyophilized)
• Lactose Enzyme Mix (Lyophilized)
• HRP (Lyophilized)
• Lactose Standard (100 nmol/µl)

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)