Product Name
Total Carbohydrate Colorimetric Assay Kit
Product Overview
In Total Carbohydrate Assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural, which react with the Developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm. The Total Carbohydrate Assay can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.
Description
Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications. Total Carbohydrate Assay is a simple, sensitive and robust method of detecting virtually all carbohydrates. The assay is based on the phenol-sulfuric acid method.
Applications
Can detect most forms of carbohydrates, including simple & complex saccharides, glycans, glycoproteins & glycolipids
Kit Components
• Assay Buffer
• Developer
• Standard (D-Glucose, 2 mg/ml)
Materials Required but Not Supplied
Concentrated H2SO4 (98%)
96-well clear plate with flat bottom
Temperature controlled Heat Block
Multi-well spectrophotometer (plate reader)
Safety goggles and gloves
Caution: H2SO4 is highly corrosive and oxidizing; handle with protective clothing, goggles and gloves etc. Do not add water to concentrated acid.
Detection method
Absorbance (490 nm)
Compatible Sample Types
Adherent or suspension cells
Animal and plant tissues
Food products, fruit juices & other beverages
Datasheet 