Product Name
Zinc Assay Kit (Fluorometric)
Product Overview
Zinc Fluorometric Assay kit provides an easy and quantitative method to measure the metal ion Zinc in various biological samples. The assay is based on the ability of our proprietary probe that binds with high specificity to Zinc and becomes fluorescent. The fluorescent intensity is directly proportional to the amount of zinc, which can be quantified by measurement at Ex/Em= 435/535 nm in a microplate reader. This assay kit provides a just add-and-read, non-radioactive, and high-throughput adaptable for Zinc detection. The assay is rapid (less than 10 min) and sensitive (Limit of Detection: <0.1 µM)
Description
Zinc (Zn, Atomic Number: 30), is a metal with characteristics similar to Magnesium due to its size and its oxidation state of +2. This element is an essential mineral of great biological significance since many enzymes require zinc as an essential cofactor. Examples of biological roles of Zinc include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function.
Applications
The kit can detect 20 ng/ml (~0.1 μM).
Kit Components
• Zinc Assay Buffer
• Zinc Probe (in DMSO)
• Zinc Standard (50 mM)
Materials Required but Not Supplied
96-well white plate with flat bottom for Fluorometric measurement
Multichannel or single channel Pipettes
Multi-well spectrophotometer (Fluorescence reader)
Detection method
Fluorescence (Ex/Em= 435/535 nm)
Compatible Sample Types
Biological Samples: Serum, Plasma, Urine, etc
Datasheet 