Product Name
Invertase Activity Colorimetric Assay Kit
Product Overview
Invertase Activity Assay kit provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.
Description
Invertase (EC 3.2.1.26), also known as sucrase or β -fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes.
Applications
Measurement of Invertase activity in various biological fluids, bacteria and crude enzyme preparations.
Analysis of metabolic pathways such as glycolysis and Diabetes Obesity-related Signaling.
Kit Components
• Invertase Hydrolysis Buffer
• Invertase Assay Buffer
• Invertase Stop Solution
• OxiRed™ Probe (in DMSO)
• Invertase Enzyme Mix
• Invertase Substrate
• Glucose Standard (100 mM)
• Positive Control
Materials Required but Not Supplied
96-well clear plate with flat bottom
Multi-well spectrophotometer
10 kDa Spin Column
Detection method
Absorbance (OD 570 nm)
Compatible Sample Types
Biological fluids e.g. saliva; Bacteria; Crude/purified enzyme preparations
Datasheet 