Product Name
NAD/NADH Quantitation Colorimetric Kit
Product Overview
The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.
Description
Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.
Applications
NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.
Kit Components
• NADH/NAD Extraction Buffer
• NAD Cycling Buffer
• NAD Cycling Enzyme Mix
• NADH Developer
• Stop Solution
• NADH Standard (MW:763)
Materials Required but Not Supplied
96-well clear plate with flat bottom
Multi-well spectrophotometer
Detection method
Absorbance (450 nm)
Compatible Sample Types
Animal tissues: liver, kidney etc.
Cell culture: adherent or suspension cells
Serum; Urine
Datasheet 