Product Name
Starch Colorimetric/Fluorometric Assay Kit
Product Overview
The Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
Description
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer). Starch generally contains 0-25% amylose and 75–100% amylopectin.
Applications
The assay can detect starch at 0.0004 to 2 mg/ml.
Kit Components
• Hydrolysis Buffer
• Development Buffer
• OxiRed Probe
• DMSO (anhydrous)
• Hydrolysis Enzyme Mix
• Development Enzyme Mix
• Starch Standard (2.0 mg/ml)
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Datasheet 