Product Name
Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit
Product Overview
The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H₂O₂which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).
Description
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.
Applications
The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.
Kit Components
• Cholesterol Assay Buffer
• Cholesterol Probe (in DMSO, anhydrous)
• Enzyme Mix (lyophilized)
• Cholesterol Esterase (lyophilized)
• Cholesterol Standard (2 µg/µl)
Materials Required but Not Supplied
96-well plate with flat bottom
Multi-well spectrophotometer
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Compatible Sample Types
Animal tissues
Cell culture: Adherent or suspension cells
Serum
Datasheet 