Phomopsis viticola End-Point PCR Detection Kit (24)

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Phomopsis viticola End-Point PCR Detection Kit (24)

Phomopsis viticola End-Point PCR Detection Kit (24) is designed for the detection of Phomopsis viticola DNA based on the use of end-point PCR technology.

Phomopsis viticola End-Point PCR Detection Kit (24) is designed for the detection of P. viticola specific DNA based on the use of end-point PCR technology. This kit is designed for research use only and not for use in diagnostic procedures. The kit includes Master Mix and primers for the specific amplification of a 300 nucleotide region of the P. viticola genome, as well as a positive control and a negative control to confirm the integrity of the kit reagents. In addition, the kit contains loading dye and a DNA ladder to facilitate analysis of the results.

All kit components should be stored at -20°C upon arrival;
Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots;
All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

Component Product
2X PCR Master Mix 350 μL
P. viticola Primer Mix 70 μL
P. viticola Positive Control 50 μL
Nuclease-Free Water 1.25 mL
Loading Dye 100 μL
DNA Ladder 100 μL
Product Insert 1

Appropriate Real-Time PCR Instrument with FAM and HEX filter channel;
DNA Purification Kit: The kit is compatible with all DNA purification kits that yield high quality, inhibitor-free DNA;
Disposable powder-free gloves;
Benchtop microcentrifuge;
Micropipettors;
Sterile pipette tips with filters;
PCR tubes;
Vortex mixer;
Agarose gel electrophoresis apparatus;
UV transilluminator with suitable gel documentation system;
PCR reaction preparation station (Optional).

Phomopsis cane and leaf spot, caused by Phomopsis viticola, occurs in most grape-growing regions and is an important disease of grapes worldwide. The fungus attacks all green parts of the vine, including canes, leaves, flowers, rachises, and berries. However, most economic damage is caused by rachis and berry infection. Infected leaves have small, yellowish spots with dark brown centers and may be puckered. With successive cool, wet springs, the inoculum will build up and infections will become more severe over time. Since symptoms appear 21 to 30 days after infection, early detection of the pathogen in the vineyard is therefore crucial for its control.

End-Point PCR

Plant tissues