Xylella fastidiosa Pathogen Detection Kits

PDB-168

Xylella fastidiosa Pathogen Detection Kits

Xylella fastidiosa PCR Kit is designed for the detection of Xylella fastidiosa specific DNA in a real-time PCR.

Xylella fastidiosa PCR Kit is designed for the detection of Xylella fastidiosa specific DNA in a real-time PCR based on the use of technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Xylella fastidiosa specific DNA is based on PCR providing a simple, reliable and rapid result for the detection of Xylella fastidiosa infection. Xylella fastidiosa PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Xylella fastidiosa PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

1. Before use, suitable amounts of all components should be completely thawed at room temperature, mixed by gentle vortexing or by pipetting, and centrifuged briefly.
2. Work quickly on ice.
3. The recommended minimum number of DNA samples tested per PCR run is 6.

Bacteria

1. Follow universal precautions. All specimens should be considered as potentially infectious and handled accordingly.
2. Ensure that a suitable lab coat, disposable gloves, and protective goggles are worn when handling specimens and kit reagents.
3. Use sterile pipette tips with filters. Use proper pipetting techniques and maintain the same pipetting pattern throughout the procedure to ensure optimal and reproducible values.
4. Ensure that appropriate specimen collection, transport, storage, and processing techniques are followed for optimal performance of this test.

1. MDx 2X PCR Master Mix 350 µL
2. Xylella fastidiosa Primer & Probe Mix 70 µL
3. Xylella fastidiosa Positive Control 50 µL
4. Nuclease-Free Water (Negative control) 1.25 mL
5. Product Insert 1

1. Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
2. DNA Purification Kit
3. Disposable powder-free gloves
4. Benchtop microcentrifuge
5. Micropipettors
6. Sterile pipette tips with filters
7. PCR tubes
8. Vortex mixer
9. PCR reaction preparation station (Optional)

Xylella fastidiosa is an Gram-negative bacteria and is transmitted by xylem fluid feeding sap insects. Diseases related with X. fastidiosa are bacterial leaf scorch, oleander leaf scorch, coffee leaf scorch (CLS), alfalfa dwarf, phony peach disease, and the economically important Pierce’s disease of grapes (PD) , citrus variegated chlorosis (CVC) and olive quick decline syndrome (OQDS) in Europe. X. fastidiosa is currently regulated in the EU as a quarantine organism. Pathogenicity of the bacterium only occurs when a large proportion of xylem vessels in a plant are colonized thus many plants are asymptomatic carriers of X. fastidiosa, which can contribute to its spread. Early and sensitive detection could prevent the spreading and help effective pest control.

PCR

The specificity of Xylella fastidiosa PCR Detection Kit is first and foremost ensured by the selection of the Xylella fastidiosa specific primers, as well as the selection of stringent reaction conditions. The Xylella fastidiosa primers were checked for possible homologies to all microorganisms in GenBank published sequences by sequence comparison analysis.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.