Penicillium sp. Detection Kits

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Penicillium sp. Detection Kits

Penicillium sp.PCR Kit is designed for the detection of Penicillium sp. specific DNA in a real-time PCR.

Penicillium sp. PCR Kit is designed for the detection of Penicillium sp. specific DNA in a real-time PCR based on the use of technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Penicillium sp. specific DNA is based on PCR providing a simple, reliable and rapid result for the detection of Penicillium sp. infection. Penicillium sp. PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Penicillium sp. PCR Kit comprises Master Mix for the target and PCR controldetection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-freewater) to confirm the integrity of the kit reagents.

1. Before use, suitable amounts of all components should be completely thawed at room temperature, mixed by gentle vortexing or by pipetting, and centrifuged briefly.
2. Work quickly on ice.
3. The recommended minimum number of DNA samples tested per PCR run is 6.

Fungi

1. Follow universal precautions. All specimens should be considered as potentially infectious and handled accordingly.
2. Ensure that a suitable lab coat, disposable gloves, and protective goggles are worn when handling specimens and kit reagents.
3. Use sterile pipette tips with filters. Use proper pipetting techniques and maintain the same pipetting pattern throughout the procedure to ensure optimal and reproducible values.
4. Ensure that appropriate specimen collection, transport, storage, and processing techniques are followed for optimal performance of this test.

1. MDx 2X PCR Master Mix 350 µL
2. Penicillium sp. Primer & Probe Mix 70 µL
3. Penicillium sp. Positive Control 50 µL
4. Nuclease-Free Water (Negative control) 1.25 mL
5. Product Insert 1

1. Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
2. DNA Purification Kit
3. Disposable powder-free gloves
4. Benchtop microcentrifuge
5. Micropipettors
6. Sterile pipette tips with filters
7. PCR tubes
8. Vortex mixer
9. PCR reaction preparation station (Optional)

Penicillium is major causative agents of food spoilage (dairy products, fruits, vegetables and meat) and postharvest decay. The genus causes significant economic losses to the fruit industry and is also of potential public health significance since Penicillium species (Penicillium sp.) produce a number of mycotoxins known to cause harmful effects in humans and animals. Penicillium is also a huge problem in the wine industry. Its presence in wine and grape juice during the various stages of fermentation is highly detrimental to the quality of the wine due to the production of compounds such as Geosmin (trans-1,10-dimethyl-trans-9-decalol) an earthy-musty compound which produces off odours and flavours. For these reasons the rapid and specific detection of Penicillium species are important for ensuring microbiological quality and safety of fruits and juices among other foods.

PCR

The specificity of Penicillium sp. PCR Detection Kit is first and foremost ensured by the selection of the Penicillium sp. -specific primers, as well as the selection of stringent reaction conditions. The Penicillium sp. primers were checked for possible homologies to all microorganisms in GenBank published sequences by sequence comparison analysis.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.