Phomopsis viticola Detection Kits

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Phomopsis viticola Detection Kits

Phomopsis viticola PCR Kit is designed for the detection of Phomopsis viticola specific DNA in a real-time PCR.

Phomopsis viticola PCR Kit is designed for the detection of Phomopsis viticola specific DNA in a real-time PCR based on the use of technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Phomopsis viticPhomopsis viticola specific DNA is based on PCR providing a simple, reliable and rapid result for the detection of Phomopsis viticola infection. Phomopsis viticola PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Phomopsis viticola PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

1. Before use, suitable amounts of all components should be completely thawed at room temperature, mixed by gentle vortexing or by pipetting, and centrifuged briefly.
2. Work quickly on ice.
3. The recommended minimum number of DNA samples tested per PCR run is 6.

Fungi

1. Follow universal precautions. All specimens should be considered as potentially infectious and handled accordingly.
2. Ensure that a suitable lab coat, disposable gloves, and protective goggles are worn when handling specimens and kit reagents.
3. Use sterile pipette tips with filters. Use proper pipetting techniques and maintain the same pipetting pattern throughout the procedure to ensure optimal and reproducible values.
4. Ensure that appropriate specimen collection, transport, storage, and processing techniques are followed for optimal performance of this test.

1. MDx 2X PCR Master Mix 350 µL
2. Phomopsis viticola Primer & Probe Mix 70 µL
3. Phomopsis viticola Positive Control 50 µL
4. Nuclease-Free Water (Negative control) 1.25 mL
5. Product Insert 1

1. Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
2. DNA Purification Kit
3. Disposable powder-free gloves
4. Benchtop microcentrifuge
5. Micropipettors
6. Sterile pipette tips with filters
7. PCR tubes
8. Vortex mixer
9. PCR reaction preparation station (Optional)

Phomopsis cane and leaf spot, caused by Phomopsis viticola, occurs in most grape-growing regions and is an important disease of grapes worldwide. The fungus attacks all green parts of the vine, including canes, leaves, flowers, rachises, and berries. However, most economic damage is caused by rachis and berry infection. Infected leaves have small, yellowish spots with dark brown centers and may be puckered. With successive cool, wet springs, the inoculum will build up and infections will become more severe over time. Since symptoms appear 21 to 30 days after infection, early detection of the pathogen in the vineyard is therefore crucial for its control.

PCR

The specificity of Phomopsis viticola PCR Detection Kit is first and foremost ensured by the selection of the Phomopsis viticola-specific primers, as well as the selection of stringent reaction conditions. The Phomopsis viticola primers were checked for possible homologies to allmicroorganisms in GenBank published sequences by sequence comparison analysis.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.