Saccharomyces cerevisiae Detection Kits

PDF-120

Saccharomyces cerevisiae Detection Kits

Saccharomyces cerevisiae PCR Kit is designed for the detection of Saccharomyces cerevisiae specific DNA in a real-time PCR.

Saccharomyces cerevisiae PCR Kit is designed for the detection of Saccharomyces cerevisiae specific DNA in a real-time PCR based on the use of technology. This kit is designed for research use only and not for use in diagnostic procedures.The detection of Saccharomyces cerevisiae specific DNA is based on PCR providing a simple, reliable and rapid result for the detection of Saccharomyces cerevisiae infection. Saccharomyces cerevisiae PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Saccharomyces cerevisiae PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

1. Before use, suitable amounts of all components should be completely thawed at room temperature, mixed by gentle vortexing or by pipetting, and centrifuged briefly.
2. Work quickly on ice.
3. The recommended minimum number of DNA samples tested per PCR run is 6.

Yeast

1. Follow universal precautions. All specimens should be considered as potentially infectious and handled accordingly.
2. Ensure that a suitable lab coat, disposable gloves, and protective goggles are worn when handling specimens and kit reagents.
3. Use sterile pipette tips with filters. Use proper pipetting techniques and maintain the same pipetting pattern throughout the procedure to ensure optimal and reproducible values.
4. Ensure that appropriate specimen collection, transport, storage, and processing techniques are followed for optimal performance of this test.

1. MDx 2X PCR Master Mix 350 µL
2. Saccharomyces cerevisiae Primer & Probe Mix 70 µL
3. Saccharomyces cerevisiae Positive Control 50 µL
4. Nuclease-Free Water (Negative control) 1.25 mL
5. Product Insert 1

1. Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
2. DNA Purification Kit
3. Disposable powder-free gloves
4. Benchtop microcentrifuge
5. Micropipettors
6. Sterile pipette tips with filters
7. PCR tubes
8. Vortex mixer
9. PCR reaction preparation station (Optional)

Saccharomyces cerevisiae plays a beneficial role in wine fermentation, in which it is the predominant species. Despite the beneficial role of S. cerevisiae in the food industry for food and beverage production, it is able to cause spoilage in wines. Uncontrolled yeast growth can alter the chemical composition of wine, detracting from its sensory properties of appearance, aroma, and flavour. If these faults are severe, the wine is rejected by consumers. S. cerevisiae contamination is found mainly in sweet wines, where fermentable sugar can support growth, and also in semidry bottled wines. Therefore a rapid and sensitive method to detect S. cerevisiae contamination is required to prevent wine spoilage.

PCR

The specificity of Saccharomyces cerevisiae PCR Detection Kit is first and foremost ensured by the selection of the Saccharomyces cerevisiae -specific primers, as well as the selection of stringent reaction conditions. The Saccharomyces cerevisiae primers were checked for possible homologies to all microorganisms in GenBank published sequences by sequence comparison analysis.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.