Listeria monocytogenes TaqMan PCR Kit

FPD-002

Listeria monocytogenes TaqMan PCR Kit

Listeria monocytogenes TaqMan PCR kit is designed for the detection of Listeria monocytogenes in food.

Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium. Due to its ability to survive high and low temperatures as well as low pH, it could resist various food processing technologies. L. monocytogenes is known to be associated with raw meat, unpasteurized milk and dairy products, vegetables, and seafood. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals being the most susceptible.

Listeria monocytogenes TaqMan PCR Kit is designed for the detection of Listeria monocytogenes-specific DNA in foods. The detection of this kit is based on TaqMan PCR, which is simple, reliable and fast. And the kit includes Master Mix, Primer & Probe Mix for target and PCR control detection, and positive and negative controls (nuclease-free water) to confirm the integrity of the kit's reagents.

Listeria monocytogenes

Each Listeria monocytogenes TaqMan PCR Kit is tested against predetermined specifications to ensure consistent product quality.

If the reagents are to be used only intermittently, they should be frozen in aliquots.

All reagents can be stored for 1 year at -20℃ without showing any reduction in performance.

This product should be stored at -20℃ upon arrival. Repeated thawing and freezing (> 2 ×) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay.

Follow general precautions. All specimens should be considered potentially infectious and handled accordingly.
1. Take protective measures.
2. Use sterile pipette tips.
3. Use aseptic technique.
4. Do not use consumables and equipment in dedicated areas for i) sample extraction, ii) reaction setup, and iii) amplification/detection.
5. Store and extract positive material (specimens, controls, and amplicons) separately from all other reagents.
6. Dispose of unused kit reagents and samples according to local, provincial or federal regulations.
7. The presence of PCR inhibitors may lead to false negative or invalid results. Potential mutations within the targeted region of the Listeria monocytogenes genome covered by the primers in this kit may result in the undetectable presence of the pathogen.
8. Ensure that proper specimen collection, transport, storage, and handling techniques are followed to achieve optimum performance of this test.

24 rxn

MDx TaqMan 2× PCR Master Mix 350 µL
Listeria monocytogenes Primer & Probe Mix 70 µL
Listeria monocytogenes Positive Control 50 µL
Nuclease-Free Water (Negative control) 1.25 mL
Product Insert 1

Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
DNA Purification Kit
Disposable powder-free gloves
Benchtop microcentrifuge
Micropipettors
Sterile pipette tips with filters
PCR tubes
Vortex mixer
PCR reaction preparation station (Optional)

Food

Ready to use format