Product Name
EthD-2 (Ethidium Homodimer-2)
Product Overview
Ethidium Homodimer-2 (EthD-2) is used for fluorescence imaging of cell nuclei.
Description
Ethidium Homodimer-2 (EthD-2) is a high-affinity fluorescent nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~535/624). The EthD-2 is highly positively charged and cell-impermeant and widely used as a cell viability indicator.
Applications
Ethidium Homodimer-2 is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. However, it is very useful to detect nucleic acids in solution, or detect dead cells with disintegrated cell membranes.
Usage
Nuclear fluorescent probe
Stability
Stable under normal conditions.
Storage
-20℃, protect from light.
Introduction
Ethidium Homodimer-2 (EthD-2) is a high affinity fluorescent nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima 535/624). The EthD-2 is highly positively charged and cell-impermeant, and widely used as cell viability indicator. EthD-2 is provided as 1 mM solution in DMSO.
Handling
Always wear recommended Personal Protective Equipment. No special handing advice is required.
Category
Plant Organelle Fluorescent Probes
Excitation/Emission (nm)
535/624
Molecular Formula
C51H60I4N8
Full Name
ethidium homodimer-2 (EthD-2)
Potential Health Hazard Effects
May cause eye irritation in susceptible persons.
May cause skin irritation in susceptible persons.
May be harmful by inhalation.
May be harmful if swallowed.
Polymerization
Hazardous polymerization does not occur.
Notice
Use the reagent as soon as possible after unpacking!
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Datasheet 