Product Name
Chloroplast Isolation Kit
Product Overview
The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves.
Applications
Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.
Usage
Chloroplast Isolation Kit
Storage
Store the kit at 2 to 8 centigrade. The Filter Mesh 100 can be stored at room temperature. It can be reused and can be autoclaved.
Introduction
The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
Kit Components
Chloroplast Isolation Buffer 5× (CIB) 500 mL
Percoll 100 mL
Bovine Serum Albumin (BSA) 3 g
Filter Mesh 100 4 each
Materials Required but Not Supplied
Scissors
Centrifuge tubes (50 mL, 14 mL)
Centrifuge (Sorvall with SS-34 rotor or equivalent)
Blender or homogenizer
Category
Plant Organelle Isolation Kits
Notice
1. Protease inhibitors can also be stored at 2-8℃ until used unopened. Store at -20℃ after use with the cap on.
2. Protease inhibitors are solid when stored at 2-8℃. After removal from the refrigerator, return to room temperature or a short water bath at 37℃, then centrifuge to the bottom of the tube before opening.
3. Use the reagent as soon as possible after unpacking!
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