Colchicine Analytical Services

Colchicine is an alkaloid extracted from the bulb and seeds of Colchicine, family Liliaceae. It is a class of natural products containing polycyclic aromatic structures with various biological activities, including inhibition of cell mitosis and anti-tumor effects. It is widely used in cytology, genetics research, and plant breeding. Colchicine is often found in complex mixtures, which makes their identification and quantification challenging. Lifeasible's team of experts has extensive experience in identifying and characterizing colchicine, providing accurate and reliable results using state-of-the-art equipment and techniques.

Analytical Methods for Colchicine

• Chromatography

Commonly used chromatographic techniques include High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). HPLC is commonly used for the separation and quantitative analysis of colchicine and can be calibrated to determine the amount present in a sample. At the same time, GC is suitable for characterizing the volatility of the different compounds in a sample.

• Mass Spectrometry

Mass spectrometry provides information on the molecular mass and structure of colchicine. Commonly used mass spectrometry techniques include mass spectrometry coupling techniques such as gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS).

• Nuclear Magnetic Resonance (NMR)

NMR techniques can provide structural information about colchicine, including the relationship between atoms and the chemical environment. By analyzing the NMR spectrum, the structure and purity of colchicine can be determined.

In addition to the above methods, colchicine can be analyzed using ultraviolet-visible spectroscopy (UV-Vis), infrared spectroscopy (IR), and mass spectroscopy (MALDI-TOF).

Colchicine Analysis

Lifeasible uses HPLC and LC-MS technologies to detect changes efficiently and accurately in colchicine content. Lifeasible has developed specialized sample preparation protocols and analytical methods to detect and quantify colchicine in these complex matrices with high precision and accuracy. In addition to detecting colchicine content, it can also analyze colchicine

• Quality Control

Quality control of colchicine detection can be used for quality control of analytical testing by calibration curve plotting, multiple testing, and performing together calibration and verification.

• Project test reports are available upon completion of analytical testing:
• Experimental steps
• Relevant mass spectrometry parameters
• Raw data
• Colchicine content information

Precautions for Colchicine Detection

Safety Precautions - Colchicine is an alkaloid and is toxic. Appropriate personal protective equipment (PPE), such as lab gloves, eyeglasses, and a lab coat, should be worn during sample handling and experimental operations to ensure personal safety.

Sample Pretreatment - Proper sample pretreatment steps are essential for the detection of colchicine. Ensure the details and conditions of the sample handling process to avoid possible interferences.

Laboratory Environmental Control - When performing colchicine testing, keep the laboratory environment clean and tidy and avoid possible cross-contamination. Pay attention to the calibration and maintenance of laboratory equipment to ensure accurate measurement and analysis.

Applications of Colchicine Assay

• Toxicity indication

The detection of colchicine allows early detection and identification of plant materials or products containing colchicine to avoid the risk of poisoning in humans and animals.

• Pharmaceutical research and development

By detecting colchicine, its content and purity can be determined, providing accurate data and information for pharmaceutical research and development.

• Botanical research

Through the detection of colchicine, the content, and changes of colchicine in plants can be assessed, thus providing insights into the ecological and physiological characteristics of plants. With state-of-the-art technology and a team of talented scientists and experts, Lifeasible is committed to providing the best alkaloid analytical services at competitive prices to customers worldwide. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.