Extraction of Endoplasmic Reticulum in Plants

The endoplasmic reticulum (ER) is a continuous membrane system consisting of the nuclear envelope, a flat peripheral sheet containing ribosomes, and interconnected tubules that run through most of the cytoplasm. The ER acts as a protein factory and calcium depot and also as an environment for monitoring protein quality, which is monitored by various signaling pathways mediated by protein folding assistants. By using the extraction method, the plant endoplasmic reticulum protein with high purity was obtained, which laid the foundation for further study of endoplasmic reticulum proteomics.

Lifeasible provides extraction services of endoplasmic reticulum in plants to help our customers worldwide in plant scientific research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Extraction Methods of Endoplasmic Reticulum in Plants

• To extract endoplasmic reticulum from leaves by sucrose density gradient centrifugation, it is necessary to prepare plant leaf homogenate, prepare sucrose density gradient solution, and finally obtain bands with different colors and widths. Endoplasmic reticulum is mainly enriched in the light brown liquid.
• Endoplasmic reticulum is extracted by differential centrifugation combined with calcium chloride precipitation. After adding CaCl₂ solution to plant leaf homogenate, the fractions of rough endoplasmic reticulum (ER) are precipitated.
• Endoplasmic reticulum is extracted by differential centrifugation, including overspeed, and the precipitate obtained is the total ER fraction by centrifugation at different speeds for different times.

Extraction Workflows of Endoplasmic Reticulum in Plants

Fig.1 Extraction of ER in plants by differential centrifugation combined with calcium chloride precipitation.

Extraction of Endoplasmic Reticulum in Plant Tissues

• Lifeasible provides extraction of ER from plant leaves; the structure of leaves is complex and contains a lot of chloroplast interference, so it is difficult to isolate and purify the endoplasmic reticulum. By optimizing the purification method, we can get purer endoplasmic reticulum in leaves.
• The root tip is essential for root growth and development and undergoes morphological and physiological changes in response to various environments. To study the function and related mechanism of the apical endoplasmic reticulum, we provide its extraction services as the basis for further research.
• In addition, we also provide the isolation services of the apical ER from seeds, internodes, and others.

Lifeasible provides cost-effective, high quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.