Product Name
BirA (mutated/TurboID) - Biotin conjugated 40 µg
Immunogen
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Background
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
Reactivity
E. coli BirA - mutated/TurboID overexpressed in various organisms
Confirmed reactivity
BirA (mutated/TurboID)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
Depends upon fusion partner
Recommended dilution
1 : 5000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Datasheet 