Product Name
Clathrin heavy-chain 1,2, ALP-conjugated (40 µg)
Immunogen
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Background
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Reactivity
Arabidopis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
Predicted reactivity
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera
Not reactive in
Nicotiana benthamiana
Expected/apparent MW
193 | 170 kDa (Arabidopsis thaliana)
Tested applications
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Datasheet 